However the yield obtained was not high, around 40% in the best case. To improve the overall yield, we have tested during a previous work several chromatography matrices (Sephacryl S200, Sephacryl S300, Sepharose 4FF.). The fractions of interest (11 in total) were pooled and checked for their antigenic activity according to the NIH potency test. The pooled harvests were purified by zonal centrifugation on a sucrose density gradient. Virus replication phase was conducted using perfusion culture mode, viral harvests obtained through the culture were clarified, inactivated by BPL (Beta-propiolactone) and then pooled. Vero cell growth and virus production were previously optimized studies were conducted in a 7-L bioreactor. The aim of this work is to compare the purity of rabies vaccine purified by zonal centrifugation and chromatographic methods, in terms of residual DNA level, host cell protein (HCP) level and the overall recovery yield.įor this purpose, Vero cells were grown under animal component free conditions, on Cytodex 1 microcarriers in VP-SFM medium. Vaccination against rabies is still the sole way to fight against the disease. Most of these victims are children under the age of 15. Infection causes tens of thousands of deaths every year, mainly in Asia and Africa. Dogs are the main source of human rabies deaths, contributing up to 99% of all rabies transmissions to humans. It can affect all mammals including humans. Time of going to press and is subject to change.Rabies is a viral zoonosis caused by negative-stranded RNA viruses of the Lyssavirus genus. The information in this catalogue is correct at the Please note that type diagrams and tables for Presetters can be found on page 12 and for split boring bar holders with reduction sleeve assemblies on page 13 - 14. Boring Holder, C4) and note the Product Code.Check your requirements against the type diagrams shown on pages 5-7.Select the Direction of Rotation required using the illustrations below and replace * in the Product Code with R or L. Main Spindle RotationLeft Hand Clamping ToolLeft Hand Cutting Toolĭirection of Rotation: Please specify Right (R) or Left (L) direction of rotationįind your Mazak machine in the tables on pages 8-12, select the toolholder style and option you require (e.g. Main Spindle RotationRight Hand Clamping ToolRight Hand Cutting Tool MAIN SPINDLE ROTATIONLEFT HAND CLAMPING UNITLEFT HAND CUTTING TOOL MAIN SPINDLE ROTATIONRIGHT HAND CLAMPING UNITRIGHT HAND CUTTING TOOL REMOVE ALL BURRS & SHARP EDGESIF IN DOUBT - ASK LS21 1JETel: #44 (0)1943 466788Fax: #44 (0)1943 850144TOLERANCES (Where not stated)No decimal place +/-0,5mmOne decimal place +/-0,1mmTwo decimal places +/-0,05mmNOTEAll dimensions are in mm unless stated.Remove all burrs and sharp edges. ScaleSimilar toCRAFTSMAN TOOLS LTDSide Copse, Pool Road, Otley,West Yorkshire,Įngland. Representative to discuss your requirements. In this catalogue, contact your local Seco
We also offer Split Reduction Sleeves for use Seco-Capto Toolholders for all leading machines. Options include turrets with two clampingĭeveloped a range of accessories to increaseīenefits of our Split and Hinged Boring Bar Quicker set-up times, off-line tool setting and
Integrated Turrets offer increased rigidity, Operation with through coolant as an option. Quality bearings ensure smooth and accurate Sealed units operate at speeds up to 6000 Toolholders we can also offer the following Products and special applications for Mazak Tools and Seco Tools can also jointly design Of model specific clamping units for most Together we have developed a wide variety Text of Seco-Capto Mazak CraftsmanĢSeco Tools are very pleased to launch its first Alongside the standard Seco-Capto™ range, Craftsman Tools and Seco Tools can also jointly design and manufacture a number of innovative products and special applications for Mazak machines. Together we have developed a wide variety of model specific clamping units for most Mazak machine tools. We have worked in close partnership with Craftsman Tools to develop the Seco- Capto™ quick change toolholders featured in this catalogue. Seco Tools are very pleased to launch its first Seco-Capto™ tool holding catalogue.